医学检验信息网 检验医学 2007-2-11 13:02:22

【摘要】 目的 用流式细胞仪(flowcytometer, FCM)检测周围血中CD₄^＋、CD₈^＋淋巴细胞，结合临床情况对HIV感染者和AIDS患者的免疫状况进行评价。方法 将抗凝全血进行白细胞分类计数，用双色荧光标记的单克隆抗体染色，经溶血和固定后，用FCM计数，从而得出CD₄^＋、CD₈^＋淋巴细胞数。结果 HIV感染者和AIDS患者的CD₄^＋淋巴细胞数都比正常人低，特别是AIDS患者，他们的CD₄^＋淋巴细胞数都低于200个/mm³，临床表现也很差。结论 FCM检测结果与临床评价高度一致，而且FCM比一般人工计数法更准确、方便、迅速，同时也证明FCM是监测HIV感染者和AIDS患者的免疫状况的最佳方法。 Analyze T lymphocyte subsets of HIV/AIDS patients by flowcytometer QIU zhifeng, WANG Aixia, CHEN Hongshan, et al. Peking Union Medical College Hospital, chinese Academy of Medical Science and PUMC, Beijing 100730 【Abstract】 Objective Usingflowcytometer (FCM) to detect CD₄^＋,CD₈^＋ lymphocytes in peripheral blood, combine with clinical symptoms to evaluate the hIV/AIDS patients′ immune state.Methods Anti-coagulated peripheral blood were obtained from 8 HIV-AIDS patients and 5 normal persons. After analyzing their total white cell counts, the whole blood was stained with two-color immunofluorescence using directly conjugated monoclonal antibody pairs, followed by lysis of red erythrocytes, fixation of lymphocytes, and analysis by flowcytometry. Multiplying the CD₄ and CD₈ percentages by the absolute number of lymphocytes obtained from the total white blood cell (WBC) count divide by the lymphocyte differential percentage, we got the CD₄, CD₈ counts.Results The hIV/AIDS patients′ CD₄ counts were lower than those of normal controls′. especially, the AIDS patients′ CD₄ counts were all below 200 cells/mm³ and their clinical symptoms were serious.Conclusion The HIV/AIDS patients' CD₄ counts are highly correlated with their clinical symptoms. the result also shows that FCM is accurate, sensitive and reliable in CD₄ count. 【Key words】 Flowcytometer CD₄^＋,CD₈^＋ lymphocytes Human immunodeficiency virus(HIV) Acquired immunodeficiency syndrome(AIDS) 自1985年我院报道第一例艾滋病(Acquired immunodeficiency syndrome, AIDS)患者以来，截至1998年底，我院已报道了51例人免疫缺陷病毒(Human immunodeficiency virus, HIV)感染者和AIDS患者。全国HIV感染疫情的日益加重，对临床诊断和治疗提出了很多新问题。其中，何时对病人进行抗病毒药物的治疗，药物疗效评价的指标，病人免疫状况的监测和预后判断等，是临床治疗中的关键问题。 HIV主要侵犯人的CD₄^＋淋巴细胞，引起其数量减少和功能上的缺陷，使机体免疫功能低下， 最终导致各种机会性感染和肿瘤。因此CD₄^＋淋巴细胞的检测在评价HIV感染者和AIDS患者的免疫状况及预后判断等方面都具有重要的作用。为此，我们用流式细胞仪对8例HIV感染者和AIDS患者的周围血进行CD₄^＋淋巴细胞计数，并结合临床情况对其免疫状况进行了评价，现将研究结果报道如下。 1 材料和方法

1.1 研究对象 研究组为8例HIV感染者和AIDS患者，对照组为5例本院年轻健康医护人员。

1.2 主要试剂 FITC-CD₃/PE-CD₄、FITC-CD₃/PE-CD₈、免洗溶血素、FITC-γ/PE-γ(同型阴性对照)、FLOW cHECK试剂(以上试剂购自法国IMMUNOTECH 公司)。

1.3 主要仪器 COULTER EPICS XL 流式细胞仪(美国COULTER公司)MEK-6108K型白细胞分类计数仪(日本NIHON KOHDEN公司)。

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