医学检验信息网 检验医学 2007-1-29 12:35:28

摘要： 目的 检测强直性脊柱炎（AS）患者关节积液与外周血树突状细胞(DC)的数量变化及成熟度。方法 利用组合性荧光抗体(CD3, CD14, CD16, CD19, CD20, CD56)设门从白细胞中选定树突状细胞，以30例健康人作对照，在流式细胞仪检测30例强直性脊柱炎患者病变关节积液和外周血CD123阳性、CD11c阳性的树突状细胞的数量。结果 AS静脉血CD11c⁺DC在白细胞所占比例（0.30±0.09%）比健康人静脉血CD11c⁺DC在白细胞所占比例（0.18±0.08%）高（t=5.459，p<0.001）; AS关节积液 CD11c⁺DC在白细胞所占比例（0.45±0.11%）比AS静脉血CD11c⁺DC在白细胞所占比例高（t=5.781，p<0.001）；AS静脉血CD123⁺DC在白细胞所占比例（0.15±0.08%）与健康人静脉血CD123⁺DC在白细胞所占比例（0.12±0.07%）没有显著性差异（t=1.546，p>0.05）; AS关节积液 CD123⁺DC在白细胞所占比例（0.23±0.08%）比AS静脉血CD123⁺DC在白细胞所占比例高（t=3.874，p<0.001）。结论 AS患者病变关节局部高、低成熟度的DC数量均有明显增多，而静脉血中主要表现为高成熟度DC增多, 因此AS患者抗原呈递水平明显高于健康人，与病变局部有关的抗原呈递明显增高。DC与AS间存在密切的联系。 关键词： 强直性脊柱炎； 树突状细胞 The Amount Change of Dendritic Cell in Joint Liquid and Vein blood of Ankylosing Spondylitis DENG Xinli, YANG Hongwei, CONG Yulong, ZHU Jian, AI Hongmei（Department of Clinical Laboratory, Chinese PLA General Hospital，Beijing 100853，China）. Abstact ： Objective To research the amount and maturity of dendritic cell(DC) in joint liquid and vein blood of ankylosing spondylitis（AS）. Methods We choose the dendritic cell from white cell by seting up the door with combined fluorescence antibody( CD 3, CD 14, CD 16, CD 19, CD 20, CD 56 ).We examine the amount of CD11c ⁺ DC and CD 123 ⁺ DC in joint liquid and vein blood of the ankylosing spondylitis patient(n=30) and normal healthes person(n=30) by flowcytometer. Results The percent of CD11c ⁺ DC in vein blood of AS( 0.30 ±0.09% ) is greater than which of normal control( 0.18 ±0.08% ) ( t = 5.459, p < 0.001). The percent of CD11c ⁺ DC in joint liquid of AS(0.45 ±0.11%) is greater than which in vein blood( t = 5.781, p < 0.001). The percent of CD 123 ⁺ DC in vein blood of AS(0.15 ±0.08%) is not greater than which of normal control(0.12 ±0.07%) (t = 1.546, p > 0.05). The percent of CD 123 ⁺ DC in joint liquid of AS(0.23 ±0.08%) is greater than which in vein blood(t = 3.874, p < 0.001). Conclusions Both high and low maturity of DC increase in joint fluid of AS. Only high maturity of DC increase in vein blood of AS. These concern the high level of antigen presentation in AS. There is some relation between DC and AS. Key words: ankylosing spondylitis dendritic cell 邓新立，男，1970年生，主治医师，硕士，主要从事流式细胞仪的临床与科研工作。email:bjdxl@163.com 强直性脊柱炎（ankylosing spondylitis, AS）是我国常见多发的风湿性疾病，大多在青少年晚期发病，患病率约为0.3%。AS的发病同机体的自身免疫密切相关。树突状细胞（Dendritic cell, DC）是目前发现的功能最强的抗原提呈细胞，在免疫反应中起着重要作用^[1]。本课题对AS外周血及关节积液中的DC进行分析，旨在初步探讨DC与AS之间的关系。 1

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